Ephrin-B2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49857
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Predicted:
Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CPKVDSKTVGQYE corresponding to internal region according to NP_004084.1.
Epitope
CPKVDSKTVGQYE - internal region
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Ephrin-B2 Antibody
![Western Blot: Ephrin-B2 Antibody [NBP1-49857]](https://resources.bio-techne.com/images/products/Ephrin-B2-Antibody-Western-Blot-NBP1-49857-img0001.jpg "Western Blot: Ephrin-B2 Antibody [NBP1-49857]")
Western Blot: Ephrin-B2 Antibody [NBP1-49857]
Western Blot: Ephrin B2 Antibody [NBP1-49857] - (0.1ug/ml) staining of Rat Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry: Ephrin-B2 Antibody [NBP1-49857]](https://resources.bio-techne.com/images/products/Ephrin-B2-Antibody-Immunohistochemistry-NBP1-49857-img0002.jpg "Immunohistochemistry: Ephrin-B2 Antibody [NBP1-49857]")
Immunohistochemistry: Ephrin-B2 Antibody [NBP1-49857]
Immunohistochemistry: Ephrin-B2 Antibody [NBP1-49857] - (A) Representative photomicrographs (left panel) and bar graph (right panel) showing AVF venous wall thickness in AVF treated with control or Ephrin-B2/Fc (day 21); *P=0.03 (t-test). n=4. Scale bar, 25um. (B) Line graph showing IVC diameter in mice treated with control or Ephrin-B2/Fc; *P<0.0001 (ANOVA). n=4-5. (C) Bar graphs showing percentage of cells in the AVF positive for proliferation (Ki67) or apoptosis (cleaved caspase-3) (day 21); *P=0.02 (t-test). n=4. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-13071-2), licensed under a CC-BY license.Applications for Ephrin-B2 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:16000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human, mouse and rat lung lysates (calculated MW of 36.9 kDa band according to NP_004084.1). Use in IHC-P reported in scientific literature (PMID: 29133876).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ephrin-B2
Alternate Names
EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1
Gene Symbol
EFNB2
UniProt
Additional Ephrin-B2 Products
Product Documents for Ephrin-B2 Antibody
Product Specific Notices for Ephrin-B2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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