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Ephrin-B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84830

Novus Biologicals, part of Bio-Techne
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NBP1-84830
NBP1-84830-25ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YRRRHRKHSPQHTTTLSLSTLATPKRSGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMP

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21539827). Use in Rat reported in scientific literature (PMID:28755882).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ephrin-B2 Antibody

Western Blot: Ephrin-B2 Antibody [NBP1-84830]

Western Blot: Ephrin-B2 Antibody [NBP1-84830]

Western Blot: Ephrin-B2 Antibody [NBP1-84830] - Colonic levels of EphB4 and ephrin-B2 proteins. Representative western blots showing the expression of EphB4, ephrin-B2 and beta-actin proteins in lysates, obtained from the colon of vehicle-treated normal mice (N) and in TNBS-treated mice administered with vehicle (CNT) or EphB4 20 ug/kg (EphB4). Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fphar.2019.00691/full), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Ephrin-B2 Antibody [NBP1-84830]

Immunocytochemistry/ Immunofluorescence: Ephrin-B2 Antibody [NBP1-84830]

Immunocytochemistry/Immunofluorescence: Ephrin-B2 Antibody [NBP1-84830] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830]

Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830]

Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human placenta shows moderate membranous positivity in endothelial cells.

Applications for Ephrin-B2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 21539827). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ephrin-B2

Ephrins are naturally divided into two structural groups. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins, which can be tyrosine phosphorylated upon receptor ligation. Class B ephrins show 33% amino acid sequence identity in their extracellular segments and 44% amino acid sequence identity in their cytoplasmic regions.

Alternate Names

EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1

Gene Symbol

EFNB2

Additional Ephrin-B2 Products

Product Documents for Ephrin-B2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ephrin-B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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