EPHX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33011

Novus Biologicals, part of Bio-Techne
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NBP1-33011
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Epoxide Hydrolase 1/EPHX1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EPHX1. The exact sequence is proprietary.

Localization

Microsome membrane, Single-pass membrane protein, Endoplasmic reticulum membrane, Single-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EPHX1 Antibody

Western Blot: EPHX1 Antibody [NBP1-33011]

Western Blot: EPHX1 Antibody [NBP1-33011]

Western Blot: EPHX1 Antibody [NBP1-33011] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EPHX1 Antibody [NBP1-33011]

Immunocytochemistry/ Immunofluorescence: EPHX1 Antibody [NBP1-33011]

Immunocytochemistry/Immunofluorescence: EPHX1 Antibody [NBP1-33011] - Analysis of methanol-fixed HeLa, using EPHX1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011]

Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011]

Immunohistochemistry-Paraffin: EPHX1 Antibody [NBP1-33011] - Mouse liver. EPHX1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
View 1 more images

Applications for EPHX1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Epoxide Hydrolase 1/EPHX1

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

EPHX, EPHX1, EPOX, Epoxide Hydratase, HYL1, MEH, MEH8, Microsomal Epoxide Hydrolase

Gene Symbol

EPHX1

Additional Epoxide Hydrolase 1/EPHX1 Products

Product Documents for EPHX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EPHX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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