EPHX2 Antibody (OTI1A6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70648

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Epoxide Hydrolase 2/EPHX2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human EPHX2 (NP_001970) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

62.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EPHX2 Antibody (OTI1A6) - Azide and BSA Free

Western Blot: EPHX2 Antibody (OTI1A6)Azide and BSA Free [NBP2-70648]

Western Blot: EPHX2 Antibody (OTI1A6)Azide and BSA Free [NBP2-70648]

Western Blot: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2.
Immunocytochemistry/ Immunofluorescence: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648]

Immunocytochemistry/ Immunofluorescence: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648]

Immunocytochemistry/Immunofluorescence: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EPHX2.
Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648]

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648]

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Human pancreas tissue using anti-EPHX2 mouse monoclonal antibody.
View 8 more images

Applications for EPHX2 Antibody (OTI1A6) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Epoxide Hydrolase 2/EPHX2

EPHX2 encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Alternate Names

Bifunctional Epoxide Hydrolase 2, CEH, EPHX2, Epoxide Hydratase 2, Epoxide Hydrolase 2, Cytosolic, SEH

Gene Symbol

EPHX2

Additional Epoxide Hydrolase 2/EPHX2 Products

Product Documents for EPHX2 Antibody (OTI1A6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EPHX2 Antibody (OTI1A6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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