Key Product Details

Species Reactivity

Primate

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LH39

Format

BSA Free

Concentration

1 mg/ml

View all Epithelial cell marker Antibodies »

Product Specifications

Immunogen

Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed in Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions.

Localization

Type I membrane protein. Two secreted forms are also produced.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Epithelial cell marker Antibody (LH39) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
LH39 precipitates a polypeptide of 185 kDa from cultured keratinocytes. Positive control(s): Cultured keratinocytes.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Epithelial cell marker

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. This antibody is potentially useful in the study of benign and malignant human vascular disorders, diseases and tumours. Associated with angiogenesis, epithelial neoplasms and conditions of tissue regeneration and repair such as wound healing.

Entrez Gene IDs

4582 (Human)

Gene Symbol

MUC1

Additional Epithelial cell marker Products

Product Documents for Epithelial cell marker Antibody (LH39) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Epithelial cell marker Antibody (LH39) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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