Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 16E4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all epithelial Sodium Channel beta Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)

Localization

Apical Cell Membrane

Specificity

Detects 87kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for epithelial Sodium Channel beta Antibody (16E4)

Western Blot: epithelial Sodium Channel beta Antibody (16E4) [NBP2-59381]

Western Blot: epithelial Sodium Channel beta Antibody (16E4) [NBP2-59381]

Western Blot: epithelial Sodium Channel beta Antibody (16E4) [NBP2-59381] - Analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 16E4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.

Applications for epithelial Sodium Channel beta Antibody (16E4)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: epithelial Sodium Channel beta

SCNN1B is a subunit of the epithelial sodium channel, ENaC. ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini. ENaC in the kidney, lung and colon plays an essential role in trans- epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Mutations in beta or gamma subunit of the epithelial sodium channel (ENaC) have been found to cause a hereditary form of human hypertension, Liddle syndrome.

Long Name

Amiloride-sensitive sodium channel subunit beta

Alternate Names

Beta-ENaC, Beta-NaCH, ENaCB, SCNEB, SCNN1B

Gene Symbol

SCNN1B

Additional epithelial Sodium Channel beta Products

Product Documents for epithelial Sodium Channel beta Antibody (16E4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for epithelial Sodium Channel beta Antibody (16E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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