Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all EPRS Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 950 to 1000 of human Glutamyl-prolyl-tRNA Synthetase using the numbering given in entry NP_004437.2 (GeneID 2058).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EPRS Antibody

Western Blot: EPRS Antibody [NBP2-22311]

Western Blot: EPRS Antibody [NBP2-22311]

Western Blot: EPRS Antibody [NBP2-22311] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. NBP2-22311 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: EPRS Antibody [NBP2-22311]

Immunohistochemistry-Paraffin: EPRS Antibody [NBP2-22311]

Immunohistochemistry-Paraffin: EPRS Antibody [NBP2-22311] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- EPRS used at a dilution of 1:1,000 (1ug/ml) Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: EPRS Antibody [NBP2-22311]

Immunoprecipitation: EPRS Antibody [NBP2-22311]

Immunoprecipitation: EPRS Antibody [NBP2-22311] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22311 used for IP at 6 ug/mg lysate. EPRS was also immunoprecipitated by rabbit anti-EPRS antibodies NBP2-22309 and NBP2-22310. For blotting immunoprecipitated EPRS, NBP2-22311 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for EPRS Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EPRS

Glutamyl Prolyl tRNA synthetase (EPRS) is a component of ARS multi complex. EPRS is released and phosphorylated by IFN stimulation and forms a complex with GAIT.

Alternate Names

bifunctional aminoacyl-tRNA synthetase, Cell proliferation-inducing gene 32 protein, DKFZp313B047, EARS, EC 6.1.1, EC 6.1.1.15, EC 6.1.1.18, GLNS, GLUPRORS, glutamate tRNA ligase, glutaminyl-tRNA synthetase, glutamyl-prolyl-tRNA synthetase, PARSproliferation-inducing gene 32 protein, proliferation-inducing protein 32, proline tRNA ligase, proline-tRNA ligase, prolyl-tRNA synthetase, QARS, QPRS

Gene Symbol

EPRS1

UniProt

NP_004437.2 (Human)

Additional EPRS Products

Product Documents for EPRS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EPRS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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