ER alpha/NR3A1 Antibody (CL1196)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34478

Novus Biologicals, part of Bio-Techne
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NBP2-34478
NBP2-34478-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1196

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ER alpha/NR3A1 Antibodies »

Product Summary for ER alpha/NR3A1 Antibody (CL1196)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTID

Epitope

YTVREAGPPAFYRPN

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ER alpha/NR3A1 Antibody (CL1196)

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of MCF7 cells using the Anti-ESR1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478]

Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human testis shows absence of immunoreactivity (negative control).
View 4 more images

Applications for ER alpha/NR3A1 Antibody (CL1196)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (CL1196)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (CL1196)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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