ER alpha/NR3A1 Antibody (ER506)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33320
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # ER506
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 2-185) of human ER alpha/NR3A1 protein (exact sequence is proprietary) (Uniprot: P03372)
Localization
Nucleus
Specificity
This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
Marker
Marker of Estrogen Dependence
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33321)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ER alpha/NR3A1 Antibody (ER506)
Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]
Western Blot: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320]
Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) [NBP2-33320] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 antibody (ER506).Applications for ER alpha/NR3A1 Antibody (ER506)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry
0.5-1.0ug/ml
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Simple Western
10 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ER alpha/NR3A1
Additional ER alpha/NR3A1 Products
Product Documents for ER alpha/NR3A1 Antibody (ER506)
Product Specific Notices for ER alpha/NR3A1 Antibody (ER506)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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