ER beta/NR3A2 Antibody (6A12)
Novus Biologicals, part of Bio-Techne | Catalog # NB200-303
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Chemotaxis, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 6A12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Amino acids 1-153 of human ER beta/NR3A2 expressed in E. coli.
Specificity
This is specific for Estrogen receptor-Beta.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ER beta/NR3A2 Antibody (6A12)
![Western Blot: ER beta/NR3A2 Antibody (6A12) [NB200-303]](https://resources.bio-techne.com/images/products/ER-beta-NR3A2-Antibody-6A12-Western-Blot-NB200-303-img0001.jpg "Western Blot: ER beta/NR3A2 Antibody (6A12) [NB200-303]")
Western Blot: ER beta/NR3A2 Antibody (6A12) [NB200-303]
Western Blot: ER beta/NR3A2 Antibody (6A12) [NB200-303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12]. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for ER beta/NR3A2 Antibody (6A12)
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Western Blot
1-5 ug/ml
Application Notes
ChIP usage reported in scientific literature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ER beta/NR3A2
Long Name
Estrogen Receptor beta
Alternate Names
ESR2, NR3A2
Entrez Gene IDs
2100 (Human)
Gene Symbol
ESR2
Additional ER beta/NR3A2 Products
Product Documents for ER beta/NR3A2 Antibody (6A12)
Product Specific Notices for ER beta/NR3A2 Antibody (6A12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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