ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33066

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Equine, Monkey, Sheep

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # ERb455

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all ER beta/NR3A2 Antibodies »

Product Specifications

Immunogen

C-terminus fragment of recombinant human ER beta/NR3A2 protein (Uniprot: Q92731)

Localization

Predominantly nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29439).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free

Western Blot: ER beta/NR3A2 Antibody (ERb455)Azide and BSA Free [NBP2-33066]

Western Blot: ER beta/NR3A2 Antibody (ERb455)Azide and BSA Free [NBP2-33066]

Western Blot: ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free [NBP2-33066] - Western Blot Analysis of human MCF-7 cell lysate using ER beta/NR3A2 Antibody (ERb455).
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free [NBP2-33066]

Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free [NBP2-33066]

Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free [NBP2-33066] - Formalin-paraffin ovarian cancer stained with Estrogen receptor beta Ab (ERb455).
Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free [NBP2-33066]

Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free [NBP2-33066]

Flow Cytometry: ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free [NBP2-33066] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Mouse Monoclonal Antibody (ERb455).
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Applications for ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ER beta/NR3A2

The human ER-

Long Name

Estrogen Receptor beta

Alternate Names

ESR2, NR3A2

Gene Symbol

ESR2

Additional ER beta/NR3A2 Products

Product Documents for ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ER beta/NR3A2 Antibody (ERb455) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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