ER beta/NR3A2 Antibody (ESR2/7006R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13876
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG2a Kappa Clone # ESR2/7006R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
C-terminus fragment of recombinant human ER beta/NR3A2 protein (Uniprot: Q92731 )
Reactivity Notes
Others not known.
Localization
Predominantly nuclear
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14205)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
Theoretical molecular weight: 53-59kDa
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
Theoretical molecular weight: 53-59kDa
Applications for ER beta/NR3A2 Antibody (ESR2/7006R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ER beta/NR3A2
Additional ER beta/NR3A2 Products
Product Documents for ER beta/NR3A2 Antibody (ESR2/7006R)
Product Specific Notices for ER beta/NR3A2 Antibody (ESR2/7006R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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