ErbB2/Her2 Antibody (511)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89187

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA Capture

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 511

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ErbB2/Her2 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ErbB2/Her2 extracellular domain (Accession#: NP_004439.2; Met 1-Thr 652).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ErbB2/Her2 Antibody (511)

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187]

Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187]

Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187] - Analysis of anti-ErbB2 on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified rabbit anti-human ErbB2. Second step staining with FITC conjugated goat anti-rabbit IgG (H+L) polyclonal antibody.
Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187]

Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187]

Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187] - Analysis of anti-ErbB2 on SK-BR3 cells. SK-BR3 cells were detached using 1X trypsin, washed, then stained with Purified Rabbit anti-Human ErbB2. Second step staining with FITC conjugated Goat anti-Rabbit IgG polyclonal antibody (BD Pharmingen (R).

Applications for ErbB2/Her2 Antibody (511)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Sandwich ELISA Capture

1:250-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody (511)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ErbB2/Her2 Antibody (511)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.