ErbB2/Her2 Antibody (ERBB2/8142R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20600
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # ERBB2/8142R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa1055-1255) of human ErbB2/Her2 encoding cytoplasmic domain (exact sequence is proprietary)
Localization
Cell surface.
Specificity
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu Its epitope is localized in the extracellular domain C-erbB-2/HER-2 is a member of the EGFR family This monoclonal antibody is specific and shows minimal cross-reaction with other members of the EGFR-family
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: SKBR-3 cells. Human breast carcinoma tissue (IHC).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for ErbB2/Her2 Antibody (ERBB2/8142R)
ErbB2/Her2 Antibody (ERBB2/8142R)
Formalin-fixed, paraffin-embedded human breast carcinoma stained with ErbB2/Her2 antibody (ERBB2/8142R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
ErbB2/Her2 Antibody (ERBB2/8142R)
Formalin-fixed, paraffin-embedded human breast carcinoma stained with ErbB2/Her2 antibody (ERBB2/8142R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for ErbB2/Her2 Antibody (ERBB2/8142R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
Additional ErbB2/Her2 Products
Product Documents for ErbB2/Her2 Antibody (ERBB2/8142R)
Product Specific Notices for ErbB2/Her2 Antibody (ERBB2/8142R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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