ErbB2/Her2 Antibody (HRB2/451)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29437
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]](https://resources.bio-techne.com/images/products/ErbB2-Her2-Antibody-HRB2-451-Flow-Cytometry-NBP2-29437-img0008.jpg "Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]")
![Protein Array: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]](https://resources.bio-techne.com/images/products/ErbB2-Her2-Antibody-HRB2-451-Protein-Array-NBP2-29437-img0010.jpg "Protein Array: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]](https://resources.bio-techne.com/images/products/ErbB2-Her2-Antibody-HRB2-451-Immunocytochemistry-Immunofluorescence-NBP2-29437-img0009.jpg "Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # HRB2/451
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human ErbB2/Her2 protein (Uniprot: IDP04626)
Localization
Cell Surface
Specificity
This monoclonal antibody is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33064)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ErbB2/Her2 Antibody (HRB2/451)
Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]](https://resources.bio-techne.com/images/products/ErbB2-Her2-Antibody-HRB2-451-Flow-Cytometry-NBP2-29437-img0012.jpg "Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]")
Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]
Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]](https://resources.bio-techne.com/images/products/ErbB2-Her2-Antibody-HRB2-451-Immunocytochemistry-Immunofluorescence-NBP2-29437-img0011.jpg "Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]")
Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).Applications for ErbB2/Her2 Antibody (HRB2/451)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-4 ug/ml
Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Application Notes
ELISA: For coating, order antibody without BSA)
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
UniProt
Additional ErbB2/Her2 Products
Product Documents for ErbB2/Her2 Antibody (HRB2/451)
Product Specific Notices for ErbB2/Her2 Antibody (HRB2/451)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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