ErbB2/Her2 Antibody (OTI4F10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01152
![Flow Cytometry: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]](https://resources.bio-techne.com/images/products/ErbB2-HER2-Antibody-4F10-Flow-Cytometry-NBP2-01152-img0001.jpg "Flow Cytometry: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]")
![Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]](https://resources.bio-techne.com/images/products/ErbB2-Her2-Antibody-OTI4F10-Western-Blot-NBP2-01152-img0010.jpg "Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry Whole-Mount
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI4F10
Concentration
1 mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
Antibody specificy evaluation by using microarray chip and WB analysis showed that this antibody cross-reacts with CDH1 recombinant protein in overexpression lysates.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
137.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ErbB2/Her2 Antibody (OTI4F10)
Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]
Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]](https://resources.bio-techne.com/images/products/ErbB2-Her2-Antibody-4F10-Immunocytochemistry-Immunofluorescence-NBP2-01152-img0008.jpg "Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]")
Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ErbB2/HER2.![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]](https://resources.bio-techne.com/images/products/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0005.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]")
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Human Kidney tissue using anti-ErbB2/HER2 mouse monoclonal antibody.![Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]](https://resources.bio-techne.com/images/products/ErbB2-HER2-Antibody-4F10-Western-Blot-NBP2-01152-img0002.jpg "Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]")
![Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]](https://resources.bio-techne.com/images/products/ErbB2-HER2-Antibody-4F10-Western-Blot-NBP2-01152-img0007.jpg "Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]](https://resources.bio-techne.com/images/products/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0003.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]](https://resources.bio-techne.com/images/products/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0004.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152]")
Applications for ErbB2/Her2 Antibody (OTI4F10)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry Whole-Mount
Reported in scientific literature (PMID:34944780)
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
Additional ErbB2/Her2 Products
Product Documents for ErbB2/Her2 Antibody (OTI4F10)
Product Specific Notices for ErbB2/Her2 Antibody (OTI4F10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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