ErbB2/Her2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84584

Novus Biologicals, part of Bio-Techne
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NBP1-84584
NBP1-84584-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ErbB2/Her2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTA

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949). Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ErbB2/Her2 Antibody

Western Blot: ErbB2/Her2 Antibody [NBP1-84584]

Western Blot: ErbB2/Her2 Antibody [NBP1-84584]

Western Blot: ErbB2/Her2 Antibody [NBP1-84584] - Analysis in human cell line SK-BR-3.
Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody [NBP1-84584]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody [NBP1-84584]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human cell line MCF7 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
View 3 more images

Applications for ErbB2/Her2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ErbB2/Her2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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