ErbB3/Her3 [p Tyr1289] Antibody (JE51-25)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32765
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE51-25
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic phosphopeptide corresponding to a region surrounding Tyrosine 1289 of Human ErbB3/Her3. (Uniprot: P21860)
Modification
p Tyr 1289
Localization
Cell membrane, secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
148 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ErbB3/Her3 [p Tyr1289] Antibody (JE51-25)
Western Blot: ErbB3/Her3 [p Tyr1289] Antibody (JE51-25) [NBP3-32765]
Western Blot: ErbB3/Her3 [p Tyr1289] Antibody (JE51-25) [NBP3-32765] - Western blot analysis of ErbB3/Her3 on different lysates with Rabbit anti-ErbB3/Her3 antibody (NBP3-32765) at 1/1,000 dilution.Lane 1: MCF7 cell lysate
Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate
Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with pp for 1 hour
Lysates/proteins at 20 ug/Lane.
Predicted band size: 148 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: ErbB3/Her3 [p Tyr1289] Antibody (JE51-25) [NBP3-32765]
Immunocytochemistry/ Immunofluorescence: ErbB3/Her3 [p Tyr1289] Antibody (JE51-25) [NBP3-32765] - Immunocytochemistry analysis of MCF7 cells serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes labeling ErbB3/Her3 with Rabbit anti-ErbB3/Her3 antibody (NBP3-32765) at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ErbB3/Her3 antibody (NBP3-32765) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
Applications for ErbB3/Her3 [p Tyr1289] Antibody (JE51-25)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ErbB3/Her3
Long Name
Receptor Tyrosine Protein Kinase ErbB3
Alternate Names
HER3
Gene Symbol
ERBB3
Additional ErbB3/Her3 Products
Product Documents for ErbB3/Her3 [p Tyr1289] Antibody (JE51-25)
Product Specific Notices for ErbB3/Her3 [p Tyr1289] Antibody (JE51-25)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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