Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # HFR1

Format

BSA Free

Concentration

1 mg/ml

View all ErbB4/Her4 Antibodies »

Product Specifications

Immunogen

Synthetic peptide sequence RSTLQHPDYLQEYST from the cytoplasmic domain of the protein (residues 1249-1264.)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Type I membrane protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for ErbB4/Her4 Antibody (HFR1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB4/Her4

Epidermal Growth Factor (EGF) affects cell growth through its interaction with a 170 kDa cell surface glycoprotein, known as the EGF receptor. Binding of EGF or TGF alpha to the receptor activates its associated kinase. Other members of the EGF receptor family include Neu (ErbB 2, HER2), ErbB 3 (HER3) and ErbB 4 (HER4). The cytoplasmic regions of EGFR and Neu are quite similar, but ErbB 3 and ErbB4 more closely resemble each other than EGFR or Neu. ErbB4 is the fourth member of class I receptor kinase family. ErbB4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47D, MDA MB 453, BT 474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF7, MDAMB 330 and MDA MB 361. Expression of ErbB4 is low or absent in some breast tumor cell lines such as MDA MB 231, MDA MB 157, MDA MB 468, and SKBR 3. Down-regulation of ErbB 4 in estrogen receptor-positive cancer cells inhibits proliferation.

Long Name

Receptor Tyrosine Protein Kinase ErbB4

Alternate Names

HER4

Entrez Gene IDs

2066 (Human); 13869 (Mouse)

Gene Symbol

ERBB4

Additional ErbB4/Her4 Products

Product Documents for ErbB4/Her4 Antibody (HFR1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ErbB4/Her4 Antibody (HFR1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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