ERCC1 Antibody (5F9U0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16521

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16521-100ul
NBP3-16521-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5F9U0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERCC1 Antibodies »

Product Summary for ERCC1 Antibody (5F9U0)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ERCC1 (P07992). MDPGKDKEGVPQPSGPPARKKFVIPLDEDEVPPGVAKPLFRSTQSLPTVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAW

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERCC1 Antibody (5F9U0)

Western Blot: ERCC1 Antibody (5F9U0) [NBP3-16521]

Western Blot: ERCC1 Antibody (5F9U0) [NBP3-16521]

Western Blot: ERCC1 Antibody (5F9U0) [NBP3-16521] - Western blot analysis of extracts of various cell lines, using ERCC1 Rabbit mAb (NBP3-16521) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Applications for ERCC1 Antibody (5F9U0)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (5F9U0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERCC1 Antibody (5F9U0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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