Key Product Details

Species Reactivity

Validated:

Human, Rat, Chinese Hamster

Cited:

Human, Hamster - Cricetulus (Chinese Hamster)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 8F1

Format

BSA Free

Concentration

1.0 mg/ml

View all ERCC1 Antibodies »

Product Specifications

Immunogen

Full length recombinant human ERCC1. [UniProt# P07992]

Reactivity Notes

Human, rat. Chinese Hamster reactivity reported in scientific literature (PMID: 17962301) Not yet tested in other species.

Localization

Nuclear.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for ERCC1 Antibody (8F1) - BSA Free

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Cyto-histologic comparison of anti-ERCC1 immunoreactivity using the protocol 8F1 CC1-mono (top) or 8F1 H2-60 (bottom). Left: Pleural effusion sediment of lung adenocarcinoma. Right: thoracic lymph node. Arrow: Lymphocyte. Arrowhead: Streak of endothelial cells. Asterisk: Tumor cell cluster. 400 A- original magnification. Image collected and cropped by Citeab from the following publication (Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10. J Clin Bioinforma (2011) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - BSA Free [NB500-704]

Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Formalin fixed paraffin embedded human tonsil stained with ERCC1 antibody.
View 2 more images

Applications for ERCC1 Antibody (8F1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:100-1:2000
Application Notes
IHC: In an ABC method, we suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Antibody can be used in Immunocytochemistry/Immunoflourescence as reported in the literature (PMID: 21961533)Western Blot was reported in scientific literature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB500-704 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERCC1

ERCC1, also known as RAD10, COFS4 and UV20, is a gene that functions in the nucleotide excision repair pathway. Specifically, ERCC1 is required for the repair of DNA lesions induced by UV light or formed by electrophilic compounds. ERCC1 is a heterodimer composed of ERCC1 and XPF/ERRC4 and is located in the nucleus. Mutations in ERCC1 result in cerebrooculofacio skeletal syndrome, as well as carcinogenesis. Recent research on DNA repair suggests that DNA damage may drive accelerated bone aging (PMID: 23281008), and that defaults in DNA repair mechanisms are prevalent in many lung cancers (PMID: 23276504).

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Entrez Gene IDs

2067 (Human); 292673 (Rat)

Gene Symbol

ERCC1

UniProt

P07992 (Human)

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (8F1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ERCC1 Antibody (8F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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