ERCC1 Antibody (CL1249)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34479

Novus Biologicals, part of Bio-Techne
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NBP2-34479
NBP2-34479-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL1249

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ERCC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LADCTLILAWSPEEAGRYLETYKAYEQKPADLLMEKLEQDFVSRVTECLTTVKSVNKTDSQTLLTTFGSLEQLIAASREDLALCPGLGPQKARRLFDVLHEPFLKVP

Epitope

KARRLFDVLH

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for ERCC1 Antibody (CL1249)

Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (CL1249) [NBP2-34479]

Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (CL1249) [NBP2-34479]

Immunocytochemistry/Immunofluorescence: ERCC1 Antibody (CL1249) [NBP2-34479] - Staining of U-2 OS cells using the Anti-ERCC1 monoclonal antibody, showing specific staining in the nuclear membrane and nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: ERCC1 Antibody (CL1249) [NBP2-34479]

Immunohistochemistry: ERCC1 Antibody (CL1249) [NBP2-34479]

Immunohistochemistry: ERCC1 Antibody (CL1249) [NBP2-34479] - Staining of human small intestine shows nuclear immunoreactivity both in glandular and lymphoid cells.
Immunohistochemistry: ERCC1 Antibody (CL1249) [NBP2-34479]

Immunohistochemistry: ERCC1 Antibody (CL1249) [NBP2-34479]

Immunohistochemistry: ERCC1 Antibody (CL1249) [NBP2-34479] - Staining of human stomach cancer shows strong nuclear immunoreactivity in tumor cells.
View 5 more images

Applications for ERCC1 Antibody (CL1249)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

UniProt

P07992 (Human)

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (CL1249)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERCC1 Antibody (CL1249)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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