Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # ERCC1 3H11

Format

BSA Free

Concentration

1 mg/ml

View all ERCC1 Antibodies »

Product Specifications

Immunogen

Full length HIS-tagged recombinant human ERCC1

Localization

Nuclear.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for ERCC1 Antibody (ERCC1 3H11) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): A431 cells or HeLa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Entrez Gene IDs

2067 (Human)

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (ERCC1 3H11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ERCC1 Antibody (ERCC1 3H11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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