ERCC1 Antibody (OTI1A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47733

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A3

Concentration

0.55 mg/ml

View all ERCC1 Antibodies »

Product Summary for ERCC1 Antibody (OTI1A3)

Immunogen

Full-length protein expressed in 293T cell transfected with human ERCC1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERCC1 Antibody (OTI1A3)

Western Blot: ERCC1 Antibody (OTI1A3) [NBP1-47733]

Western Blot: ERCC1 Antibody (OTI1A3) [NBP1-47733]

Western Blot: ERCC1 Antibody (1A3) [NBP1-47733] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Immunocytochemistry/Immunofluorescence: ERCC1 Antibody (1A3) [NBP1-47733] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Immunohistochemistry-Paraffin: ERCC1 Antibody (OTI1A3) [NBP1-47733]

Immunohistochemistry-Paraffin: ERCC1 Antibody (OTI1A3) [NBP1-47733]

Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded thyroid tissue using anti-ERCC1 mouse monoclonal antibody.
View 6 more images

Applications for ERCC1 Antibody (OTI1A3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.55 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERCC1

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Entrez Gene IDs

2067 (Human)

Gene Symbol

ERCC1

UniProt

P07992 (Human)

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (OTI1A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERCC1 Antibody (OTI1A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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