ERCC1 Antibody (S08-7E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14929

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14929-100ul
NBP3-14929-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-7E3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERCC1 Antibodies »

Product Summary for ERCC1 Antibody (S08-7E3)

Immunogen

A synthetic peptide of human ERCC1 (Uniprot#: P07992)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERCC1 Antibody (S08-7E3)

Western Blot: ERCC1 Antibody (S08-7E3) [NBP3-14929]

Western Blot: ERCC1 Antibody (S08-7E3) [NBP3-14929]

Western Blot: ERCC1 Antibody (S08-7E3) [NBP3-14929] - Western blot detection of ERCC1 in K562, 3T3, Hela cell lysates using ERCC1 Rabbit mAb (1:1000 diluted). Predicted band size: 33kDa. Observed band size: 39kDa.
Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (S08-7E3) [NBP3-14929]

Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (S08-7E3) [NBP3-14929]

Immunocytochemistry/Immunofluorescence: ERCC1 Antibody (S08-7E3) [NBP3-14929] - Immunofluorescence of ERCC1 (green) in Hela cells using ERCC1 Rabbit mAb at dilution 1/200, and DAPI (blue)

Applications for ERCC1 Antibody (S08-7E3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/100

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (S08-7E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERCC1 Antibody (S08-7E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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