ERF Antibody (3F11)
Novus Biologicals, part of Bio-Techne | Catalog # H00002077-M02
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 3F11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ERF (AAH22231, 181 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LGRGSVSDCSDGTSELEEPLGEDPRARPPGPPDLGAFRGPPLARLPHDPGVFRVYPRPRGGPEPLSPFPVSPLAGPGSLLPPQLSPALPMTPTHLAYTPSPTLSPMYPSG
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
ERF - Ets2 repressor factor (3F11)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ERF Antibody (3F11)
![Western Blot: ERF Antibody (3F11) [H00002077-M02]](https://resources.bio-techne.com/images/products/ERF-Antibody-3F11-Western-Blot-H00002077-M02-img0005.jpg "Western Blot: ERF Antibody (3F11) [H00002077-M02]")
Western Blot: ERF Antibody (3F11) [H00002077-M02]
Western Blot: ERF Antibody (3F11) [H00002077-M02] - ERF monoclonal antibody (M02), clone 3F11. Analysis of ERF expression in Raw 264.7.![Immunocytochemistry/ Immunofluorescence: ERF Antibody (3F11) [H00002077-M02]](https://resources.bio-techne.com/images/products/ERF-Antibody-3F11-Immunocytochemistry-Immunofluorescence-H00002077-M02-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ERF Antibody (3F11) [H00002077-M02]")
Immunocytochemistry/ Immunofluorescence: ERF Antibody (3F11) [H00002077-M02]
Immunocytochemistry/Immunofluorescence: ERF Antibody (3F11) [H00002077-M02] - Analysis of monoclonal antibody to ERF on HeLa cell. Antibody concentration 10 ug/ml.Applications for ERF Antibody (3F11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ERF
Alternate Names
Ets2 repressor factorPE-2PE2ETS domain-containing transcription factor ERF
Entrez Gene IDs
2077 (Human)
Gene Symbol
ERF
UniProt
Additional ERF Products
Product Documents for ERF Antibody (3F11)
Product Specific Notices for ERF Antibody (3F11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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