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ERG Antibody (ERG/22R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08916

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ERG/22R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide corresponding to residues within aa450 to C-terminus of ERG was used as an immunogen. (Uniprot: P11308)

Localization

Nucleus.

Marker

Vascular Endothelial & Prostate Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07173).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ERG Antibody (ERG/22R) - Azide and BSA Free

Immunohistochemistry-Paraffin: ERG Antibody (ERG/22R) - Azide and BSA Free [NBP3-08916]

Immunohistochemistry-Paraffin: ERG Antibody (ERG/22R) - Azide and BSA Free [NBP3-08916]

Immunohistochemistry-Paraffin: ERG Antibody (ERG/22R) - Azide and BSA Free [NBP3-08916] - Formalin-fixed, paraffin-embedded human prostate tissue stained with ERG Recombinant Rabbit Monoclonal Antibody (ERG/22R) at 1ug/ml. HIER: Tris/EDTA pH 9; 95C/45min. 2 Ab: HRP-Polymer:30mins; DAB:5min.
Immunohistochemistry-Paraffin: ERG Antibody (ERG/22R) - Azide and BSA Free [NBP3-08916]

Immunohistochemistry-Paraffin: ERG Antibody (ERG/22R) - Azide and BSA Free [NBP3-08916]

Immunohistochemistry-Paraffin: ERG Antibody (ERG/22R) - Azide and BSA Free [NBP3-08916] - Formalin-fixed, paraffin-embedded human brain. Negative tissue control stained with ERG antibody (ERG/22R) at 1ug/ml for 30 min at RT. HIER: Tris/EDTA pH 9; 95C/45min. 2 Ab: HRP-Polymer:30mins; DAB:5min.

Applications for ERG Antibody (ERG/22R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Erg

"ERG is a transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

Long Name

v-Ets Erythroblastosis Virus E26 Oncogene Homolog

Alternate Names

Erg-3, p55

Gene Symbol

ERG

Additional Erg Products

Product Documents for ERG Antibody (ERG/22R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ERG Antibody (ERG/22R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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