ERK1 Antibody (0B0C1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15776
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0B0C1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ERK1 Antibody (0B0C1)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERK1 (P27361). MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ERK1 Antibody (0B0C1)
![Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]](https://resources.bio-techne.com/images/products/ERK1-Antibody-0B0C1-Immunohistochemistry-Paraffin-NBP3-15776-img0002.jpg "Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]")
Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]
Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776] - Immunohistochemistry of paraffin-embedded human lung cancer using ERK1 Rabbit mAb (NBP3-15776) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]](https://resources.bio-techne.com/images/products/ERK1-Antibody-0B0C1-Immunohistochemistry-Paraffin-NBP3-15776-img0001.jpg "Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]")
Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]
Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776] - Immunohistochemistry of paraffin-embedded human colon carcinoma using ERK1 Rabbit mAb (NBP3-15776) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776]](https://resources.bio-techne.com/images/products/ERK1-Antibody-0B0C1-Immunoprecipitation-NBP3-15776-img0004.jpg "Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776]")
Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776]
Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug ERK1 antibody (NBP3-15776). Western blot was performed from the immunoprecipitate using ERK1 antibody (NBP3-15776) at a dilition of 1:1000.Applications for ERK1 Antibody (0B0C1)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ERK1
Long Name
Extracellular Signal-regulated Kinase 1
Alternate Names
MAPK3, P44ERK1, p44mapk, PRKM3
Gene Symbol
MAPK3
Additional ERK1 Products
Product Documents for ERK1 Antibody (0B0C1)
Product Specific Notices for ERK1 Antibody (0B0C1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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