ERK1 Antibody (0B0C1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15776
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0B0C1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERK1 (P27361). MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ERK1 Antibody (0B0C1)
ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using ERK1 antibody 19561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using ERK1 antibody 19561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] -
Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for ERK1 Antibody (0B0C1)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:100 - 1:800
Immunohistochemistry
1:200 - 1:2000
Immunohistochemistry-Paraffin
1:200 - 1:2000
Immunoprecipitation
5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:1000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ERK1
Long Name
Extracellular Signal-regulated Kinase 1
Alternate Names
MAPK3, P44ERK1, p44mapk, PRKM3
Gene Symbol
MAPK3
Additional ERK1 Products
Product Documents for ERK1 Antibody (0B0C1)
Product Specific Notices for ERK1 Antibody (0B0C1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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