ERK1 Antibody (0B0C1)

Name: ERK1 Antibody (0B0C1)
Brand: Novus Biologicals
SKU: NBP3-15776

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15776

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0B0C1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERK1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERK1 (P27361). MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERK1 Antibody (0B0C1)

Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776]

Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug ERK1 antibody (NBP3-15776). Western blot was performed from the immunoprecipitate using ERK1 antibody (NBP3-15776) at a dilition of 1:1000.

ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using ERK1 antibody 19561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of extracts from wild type(WT) and ERK1 knockout (KO) 293T cells, using ERK1 antibody 19561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (0B0C1) [ERK1] -

Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (0B0C1) [ERK1] - Confocal imaging of MCF7 cells using [KD Validated] ERK1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] -

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] -

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] -

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KD Validated] ERK1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (0B0C1) [ERK1] -

Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (0B0C1) [ERK1] - Confocal imaging of paraffin-embedded 5xFAD mouse brain tissue using [KD Validated] ERK1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

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Applications for ERK1 Antibody (0B0C1)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK1

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Gene Symbol

MAPK3

Additional ERK1 Products

Product Documents for ERK1 Antibody (0B0C1)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for ERK1 Antibody (0B0C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
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ERK1 Antibody (0B0C1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15776

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all ERK1 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for ERK1 Antibody (0B0C1)

Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776]

ERK1 Antibody (0B0C1) [NBP3-15776] -Analysis of various lysates, using ERK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (0B0C1) [ERK1] -

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] -

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] -

Immunohistochemistry: ERK1 Antibody (0B0C1) [ERK1] -

Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (0B0C1) [ERK1] -

Applications for ERK1 Antibody (0B0C1)

Immunohistochemistry

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: ERK1

Long Name

Alternate Names

Gene Symbol

Additional ERK1 Products

Product Documents for ERK1 Antibody (0B0C1)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for ERK1 Antibody (0B0C1)