ERK1 [p Thr202, p Tyr204] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-141

Novus Biologicals, part of Bio-Techne
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NB500-141
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Avian - Chicken

Predicted:

Bovine (100%), Canine (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERK1 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding ERK1 conjugated to KLH. Accession # P63086

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 31400551).

Modification

p Thr202, p Tyr204

Localization

Cell Membrane

Specificity

Specific for endogenous levels of the ~42 - 44 kDa ERK1 protein phosphorylated at Thr202 and Tyr204. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK1 [p Thr202, p Tyr204] Antibody

Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141]

Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141]

Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141]

Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141]

Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.

Applications for ERK1 [p Thr202, p Tyr204] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:250

Immunohistochemistry

1:500

Western Blot

1:1000
Application Notes
NB500-141 can be used in Western blot where a band is seen at ~ 42-44 kDa, representing ERK 1/2. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 26232338)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK1

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine.

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Entrez Gene IDs

5595 (Human); 26417 (Mouse); 50689 (Rat)

Gene Symbol

MAPK3

UniProt

P21708 (Rat)

Additional ERK1 Products

Product Documents for ERK1 [p Thr202, p Tyr204] Antibody

Product Datasheets

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Product Specific Notices for ERK1 [p Thr202, p Tyr204] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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