ERK2 Antibody (10C7)

Immunohistochemistry: ERK2 Antibody (10C7) [NBP3-26150] -

Immunohistochemistry: ERK2 Antibody (10C7) [NBP3-26150] - Image of ERK2 Antibody (10C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: ERK2 Antibody (10C7) [NBP3-26150] -

Western Blot: ERK2 Antibody (10C7) [NBP3-26150] - Positive Western Blot detected in: Jurkat whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A375 whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate.
All lanes: ERK2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 42, 37 kDa
Observed band size: 42 kDa

Immunocytochemistry/Immunofluorescence: ERK2 Antibody (10C7) [NBP3-26150] -

Immunocytochemistry/Immunofluorescence: ERK2 Antibody (10C7) [NBP3-26150] - Staining of Hela Cells with ERK2 Antibody (10C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).