Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6E5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ERK2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK2 Antibody (OTI6E5) - Azide and BSA Free

Western Blot: ERK2 Antibody (OTI6E5)Azide and BSA Free [NBP2-71233]

Western Blot: ERK2 Antibody (OTI6E5)Azide and BSA Free [NBP2-71233]

Western Blot: ERK2 Antibody (OTI6E5) - Azide and BSA Free [NBP2-71233] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK2.
Immunohistochemistry: ERK2 Antibody (OTI6E5) - Azide and BSA Free [NBP2-71233]

Immunohistochemistry: ERK2 Antibody (OTI6E5) - Azide and BSA Free [NBP2-71233]

Immunohistochemistry: ERK2 Antibody (OTI6E5) - Azide and BSA Free [NBP2-71233] - Staining of paraffin-embedded pancreas tissue using anti-ERK2 mouse monoclonal antibody.
Western Blot: ERK2 Antibody (OTI6E5)Azide and BSA Free [NBP2-71233]

Western Blot: ERK2 Antibody (OTI6E5)Azide and BSA Free [NBP2-71233]

Western Blot: ERK2 Antibody (OTI6E5) - Azide and BSA Free [NBP2-71233] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.
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Applications for ERK2 Antibody (OTI6E5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Immunoprecipitation

2-4ug/mg

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK2

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Gene Symbol

MAPK1

Additional ERK2 Products

Product Documents for ERK2 Antibody (OTI6E5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK2 Antibody (OTI6E5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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