ERK3/MAPK6 Antibody (1G6)
Novus Biologicals, part of Bio-Techne | Catalog # H00005597-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1G6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MAPK6 (AAH35492, 612 a.a. ~ 721 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN
Specificity
MAPK6 - mitogen-activated protein kinase 6
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ERK3/MAPK6 Antibody (1G6)
![Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]](https://resources.bio-techne.com/images/products/ERK3-MAPK6-Antibody-1G6-Western-Blot-H00005597-M01-img0008.jpg "Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]")
Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]
Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]](https://resources.bio-techne.com/images/products/ERK3-MAPK6-Antibody-1G6-Immunocytochemistry-Immunofluorescence-H00005597-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]")
Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]
Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of monoclonal antibody to MAPK6 on HeLa cell . Antibody concentration 10 ug/ml.![Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]](https://resources.bio-techne.com/images/products/ERK3-MAPK6-Antibody-1G6-Western-Blot-H00005597-M01-img0006.jpg "Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]")
Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01]
Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi ( Cat # H00005597-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 (Cat # H00005597-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for ERK3/MAPK6 Antibody (1G6)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ERK3
Long Name
Extracellular Signal-regulated Kinase 3
Alternate Names
MAPK6, p97MAPK
Entrez Gene IDs
5597 (Human)
Gene Symbol
MAPK6
OMIM
602904 (Human)
UniProt
Additional ERK3 Products
Product Documents for ERK3/MAPK6 Antibody (1G6)
Product Specific Notices for ERK3/MAPK6 Antibody (1G6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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