Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Canine, Equine, Sheep

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V23P3C9*H2

Format

BSA Free

Concentration

1 mg/ml

View all ERO1L alpha Antibodies »

Product Specifications

Immunogen

Peptide Sequence - KLIANMPESG (peptide immunogen is identical in rat (Rattus norvegicus), mouse (Mus musculus), sheep (Ovis aries), bovine (Bos taurus), canine (Canis familiaris), porcine (Sus scrofa) and equine (Equus caballus)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ERO1L Antibody (V23P3C9*H2) - BSA Free

Western Blot: ERO1L Antibody (V23P3C9*H2)BSA Free [NBP2-50388]

Western Blot: ERO1L Antibody (V23P3C9*H2)BSA Free [NBP2-50388]

Western Blot: ERO1L Antibody (V23P3C9-H2) [NBP2-50388] - Analysis of ERO1L in Jurkat cell lysate.

Applications for ERO1L Antibody (V23P3C9*H2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): ELISA - Peptide immunogen; WB - Jurkat whole cell lysate; IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERO1L alpha

Perhaps the most distinctive feature of protein folding in the ER is the abundance of disulfide bonds that must form during maturation of proteins traveling along the secretory pathway. Formation of disulfide bonds is a redox reaction. Thus, to match the flux of disulfide bonds that exit from the ER by virtue of protein secretion, a flux of oxidizing equivalents into the ER is required. In eukaryotic cells, the essential protein relay supporting this flux, and hence disulfide bond formation, involves endoplasmic reticulum oxidoreductin 1 (Ero1) and protein disulfide isomerase (PDI). The temporal pattern of hypoxic ERO1-L alpha induction is very similar to that of genes triggered by the hypoxia inducible transcription factor (HIF-1) and is characteristically mimicked by cobalt and by deferoxamine, but is absent in cells with a defective aryl hydrocarbon receptor translocator (ARNT, HIF-1 alpha). We speculate from these findings that the expression of ERO1-L alpha is probably regulated via the HIF-pathway and thus belongs to the family of classic oxygen regulated genes.

Long Name

Endoplasmic Reticulum Oxidoreductin 1 alpha

Alternate Names

ERO1-alpha, ERO1-L, ERO1-l alpha, ERO1-like, ERO1-like alpha, ERO1A, ERO1L

Gene Symbol

ERO1A

Additional ERO1L alpha Products

Product Documents for ERO1L Antibody (V23P3C9*H2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ERO1L Antibody (V23P3C9*H2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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