Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M37-P5D11

Format

BSA Free

Concentration

1 mg/ml

View all ERO1LB Antibodies »

Product Specifications

Immunogen

Peptide - LAPSRGEDDG

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ERO1LB Antibody (M37-P5D11) - BSA Free

Western Blot: ERO1LB Antibody (M37-P5D11)BSA Free [NBP2-50389]

Western Blot: ERO1LB Antibody (M37-P5D11)BSA Free [NBP2-50389]

Western Blot: ERO1LB Antibody (M37-P5D11) [NBP2-50389] - Shows detection of a 62 kDa and 49 kDa band in a Jurkat cell lysate.
Immunohistochemistry-Paraffin: ERO1LB Antibody (M37-P5D11) - BSA Free [NBP2-50389]

Immunohistochemistry-Paraffin: ERO1LB Antibody (M37-P5D11) - BSA Free [NBP2-50389]

Immunohistochemistry-Paraffin: ERO1LB Antibody (M37-P5D11) [NBP2-50389] - Immunohistochemistry images are showing a stronger cytoplasmic staining of ERO1LB in (A) primary colorectal cancer compared to (B) normal colon mucosa using ERO1LB Antibody (M37-P5D11). IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).
Immunohistochemistry: ERO1LB Antibody (M37-P5D11) - BSA Free [NBP2-50389]

Immunohistochemistry: ERO1LB Antibody (M37-P5D11) - BSA Free [NBP2-50389]

Immunohistochemistry: ERO1LB Antibody (M37-P5D11) [NBP2-50389] - Staining of a cross section from breast cancer tissue.

Applications for ERO1LB Antibody (M37-P5D11) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): WB - Jurkat whole cell lysate, IHC - formalin-fixed, paraffin-embedded human breast cancer.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERO1LB

Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress

Alternate Names

DKFZp779C1042, DKFZp779I0141, EC 1.8.4, EC 1.8.4.-, Endoplasmic oxidoreductin-1-like protein B, ERO1-L(beta), ERO1-L-beta, ERO1-like beta (S. cerevisiae), ERO1-like protein beta, FLJ11003, oxidoreductin-1-L-beta

Gene Symbol

ERO1B

Additional ERO1LB Products

Product Documents for ERO1LB Antibody (M37-P5D11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ERO1LB Antibody (M37-P5D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.