ERP44 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97000
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human ERP44 (Accession#: NP_055866.1; Met1-Asp402)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ERP44 Antibody
Western Blot: ERP44 Antibody [NBP2-97000]
Western Blot: ERP44 Antibody [NBP2-97000] - Anti-ERP44 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lane B: NCI-H1299 Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDaImmunoprecipitation: ERP44 Antibody [NBP2-97000]
Immunoprecipitation: ERP44 Antibody [NBP2-97000] - ERP44 was immunoprecipitated using: Lane A: 0.5 mg Raji Whole Cell Lysate. 2 ul anti-ERP44 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-ERP44 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDaApplications for ERP44 Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
0.5-2 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ERP44
Alternate Names
endoplasmic reticulum protein 44, endoplasmic reticulum resident protein 44 kDa, ER protein 44, ERp44, KIAA0573endoplasmic reticulum resident protein 44, PDIA10, protein disulfide isomerase family A, member 10, thioredoxin domain containing 4 (endoplasmic reticulum), Thioredoxin domain-containing protein 4, TXNDC4
Gene Symbol
ERP44
Additional ERP44 Products
Product Documents for ERP44 Antibody
Product Specific Notices for ERP44 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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