ERp57/PDIA3 Antibody (9S9X4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16394

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16394-100ul
NBP3-16394-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9S9X4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERp57/PDIA3 Antibodies »

Product Summary for ERp57/PDIA3 Antibody (9S9X4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ERp57/PDIA3 (P30101). YPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERp57/PDIA3 Antibody (9S9X4)

Western Blot: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394]

Western Blot: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394]

Western Blot: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394] - Western blot analysis of extracts of various cell lines, using ERp57/PDIA3 Rabbit mAb (NBP3-16394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (9S9X4) [NBP3-16394] - Immunohistochemistry of paraffin-embedded human placenta using ERp57/PDIA3 Rabbit mAb (NBP3-16394) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for ERp57/PDIA3 Antibody (9S9X4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERp57/PDIA3

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Long Name

Endoplasmic Reticulum Resident Protein 57

Alternate Names

ERp60, GRP58, PDIA3

Gene Symbol

PDIA3

Additional ERp57/PDIA3 Products

Product Documents for ERp57/PDIA3 Antibody (9S9X4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERp57/PDIA3 Antibody (9S9X4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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