ERp57/PDIA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29116

Novus Biologicals, part of Bio-Techne
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NBP3-29116
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERp57/PDIA3 Antibodies »

Product Specifications

Immunogen

Between 25 and 75

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERp57/PDIA3 Antibody

ERp57/PDIA3 Antibody

Immunohistochemistry;ERp57/PDIA3 Antibody[NBP3-29115]

Immunohistochemistry;ERp57/PDIA3 Antibody[NBP3-29115]Detection of human PDIA3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PDIA3 used at a dilution of 1:5,000 . Detection: DAB. Counterstain:IHC Hematoxylin .

Applications for ERp57/PDIA3 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ERp57/PDIA3

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Long Name

Endoplasmic Reticulum Resident Protein 57

Alternate Names

ERp60, GRP58, PDIA3

Gene Symbol

PDIA3

Additional ERp57/PDIA3 Products

Product Documents for ERp57/PDIA3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ERp57/PDIA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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