Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54537
![Western Blot: Erythropoietin/EPO Antibody (EPO/1368)Azide and BSA Free [NBP2-54537]](https://resources.bio-techne.com/images/products/Erythropoietin-EPO-Antibody-EPO-1368-Azide-and-BSA-Free-Western-Blot-NBP2-54537-img0002.jpg "Western Blot: Erythropoietin/EPO Antibody (EPO/1368)Azide and BSA Free [NBP2-54537]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # EPO/1368
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 28-162) of human Erythropoietin/EPO protein (exact sequence is proprietary) (Uniprot: P01588)
Localization
Cytoplasmic and Cell Surface
Marker
Marker of Placentation Disorders
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53392).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free
Western Blot: Erythropoietin/EPO Antibody (EPO/1368)Azide and BSA Free [NBP2-54537]
Western Blot: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537] - Human Heart and HepG2 Cell Lysate using EPO Monoclonal Antibody![Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537]](https://resources.bio-techne.com/images/products/Erythropoietin-EPO-Antibody-EPO-1368-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54537-img0004.jpg "Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537]")
Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537]
Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Erythropoietin/EPO Antibody (EPO/1368).![Protein Array: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537]](https://resources.bio-techne.com/images/products/Erythropoietin-EPO-Antibody-EPO-1368-Azide-and-BSA-Free-Protein-Array-NBP2-54537-img0003.jpg "Protein Array: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537]")
Protein Array: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537]
Protein Array: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Erythropoietin/EPO Antibody (EPO/1368).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.Applications for Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
4 - 8 ug/ml
Western Blot
0.5 - 1 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Erythropoietin/EPO
Alternate Names
ECYT5, EPO, MVCD2
Gene Symbol
EPO
Additional Erythropoietin/EPO Products
Product Documents for Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free
Product Specific Notices for Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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