Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Erythropoietin R Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human EPO receptor protein (between residues 300-400) [UniProt P19235]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34552120).

Localization

Membrane

Specificity

EpoR (A364) detects endogenous levels of EpoR protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Erythropoietin R Antibody - BSA Free

Western Blot: Erythropoietin R AntibodyBSA Free [NBP1-19388]

Western Blot: Erythropoietin R AntibodyBSA Free [NBP1-19388]

Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 (left) and SH-SY5Y (right) cell lysate using EPO receptor antibody at a concentration of 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Erythropoietin R Antibody - BSA Free [NBP1-19388]

Immunocytochemistry/ Immunofluorescence: Erythropoietin R Antibody - BSA Free [NBP1-19388]

Immunocytochemistry/Immunofluorescence: Erythropoietin R Antibody [NBP1-19388] - EPO receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: Erythropoietin R Antibody - BSA Free [NBP1-19388]

Immunohistochemistry-Paraffin: Erythropoietin R Antibody - BSA Free [NBP1-19388]

Immunohistochemistry-Paraffin: Erythropoietin R Antibody [NBP1-19388] - IHC analysis of the EPO receptor in human placenta.
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Applications for Erythropoietin R Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50-1:100

Simple Western

1:10

Western Blot

2.0 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-19388 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Erythropoietin R

The erythropoietin receptor (EPOR) is a cytokine receptor family member. There are three known isoforms of the receptor, EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). When bound to erythropoietin (EPO), EPOR is phosphorylated by Jak2 tyrosine kinase. Phosphorylated EPOR serves as the docking site for a number of different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. EPOR-Stat5 signaling appears to play a role in erythroid cell survival. Animal studies have shown that truncated EPOR that can still be bound by Jak2 are viable, suggesting that basal Jak2 activity is sufficient for erythropoiesis Stat5 binding is important, as a mutated Tyr343 on EPOR results in anemia and poor response to hypoxic stress.

Long Name

Erythropoietin Receptor

Alternate Names

EpoR

Entrez Gene IDs

2057 (Human); 13857 (Mouse); 24336 (Rat)

Gene Symbol

EPOR

UniProt

P19235

Additional Erythropoietin R Products

Product Documents for Erythropoietin R Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Erythropoietin R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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