Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoblotting, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ESCO2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human establishment of cohesion 1 homolog 2 using the numbering given in entry NP_001017420.1 (GeneID 157570).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ESCO2 Antibody

Western Blot: ESCO2 Antibody [NB100-87021]

Western Blot: ESCO2 Antibody [NB100-87021]

Western Blot: ESCO2 Antibody [NB100-87021] - Detection of Human ESCO2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ESCO2 Antibody [NB100-87021]

Immunoprecipitation: ESCO2 Antibody [NB100-87021]

Immunoprecipitation: ESCO2 Antibody [NB100-87021] - Detection of human ESCO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 (lot 2) used for IP at 6 ug per reaction. ESCO2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-ESCO2 antibody from Company B. For blotting immunoprecipitated ESCO2, NB100-87021 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ESCO2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ESCO2

This gene encodes a protein that may have acetyltransferase activity and may be required for the establishment of sister chromatid cohesion during the S phase of mitosis. Mutations in this gene have been associated with Roberts syndrome. [provided by RefSeq]

Alternate Names

2410004I17Rik, EC 2.3.1.-, ECO1 homolog 2, EFO2, Establishment of cohesion 1 homolog 2, establishment of cohesion 1 homolog 2 (S. cerevisiae), N-acetyltransferase ESCO2, RBS, Roberts syndrome

Entrez Gene IDs

157570 (Human)

Gene Symbol

ESCO2

UniProt

Q56NI9 (Human)

Additional ESCO2 Products

Product Documents for ESCO2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ESCO2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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