ESRP1/2 Antibody (23A7.C9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77971
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 23A7.C9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ESRP1/2 Antibody (23A7.C9) was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein. (Uniprot: Q8K0G8)
Reactivity Notes
A BLAST analysis of the immunizing protein sequence shows 100% homology with ESRP1/2 from mouse and a 91% sequence homology with ESRP1/2 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
Use in Human reported in scientific literature (PMID: 33500360)
Use in Human reported in scientific literature (PMID: 33500360)
Specificity
This antibody reacts with both mouse ESRP1/2 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with ESRP1/2 from mouse and a 91% sequence homology with ESRP1/2 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody was purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for ESRP1/2 Antibody (23A7.C9)
![Western Blot: ESRP1/2 Antibody (23A7.C9) [NBP1-77971]](https://resources.bio-techne.com/images/products/ESRP1-2-Antibody-23A7-C9-Western-Blot-NBP1-77971-img0003.jpg "Western Blot: ESRP1/2 Antibody (23A7.C9) [NBP1-77971]")
Western Blot: ESRP1/2 Antibody (23A7.C9) [NBP1-77971]
Western Blot: ESRP1/2 Antibody (23A7.C9) [NBP1-77971] - 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.Applications for ESRP1/2 Antibody (23A7.C9)
Application
Recommended Usage
ELISA
1:750000
Western Blot
1:1000
Application Notes
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 and 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: ESRP1/2
Alternate Names
epithelial splicing regulatory protein 2,9530027K23Rik, Rbm35b, RNA binding motif protein 35b, RNA-binding motif protein 35B, RNA-binding protein 35B
Gene Symbol
ESRP1
UniProt
Additional ESRP1/2 Products
Product Documents for ESRP1/2 Antibody (23A7.C9)
Product Specific Notices for ESRP1/2 Antibody (23A7.C9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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