ESRP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76536

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ESRP1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RBM35A. The immunogen is located within amino acids 30 - 80 of RBM35A. Amino Acid Squence: LVRPDQLELTED

Specificity

RBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist; this antibody will detect all five.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ESRP1 Antibody - BSA Free

Western Blot: ESRP1 AntibodyBSA Free [NBP1-76536]

Western Blot: ESRP1 AntibodyBSA Free [NBP1-76536]

Western Blot: ESRP1 Antibody [NBP1-76536] - Analysis of RBM35A in rat colon tissue lysate with RBM35A antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry-Paraffin: ESRP1 Antibody - BSA Free [NBP1-76536]

Immunohistochemistry-Paraffin: ESRP1 Antibody - BSA Free [NBP1-76536]

Immunohistochemistry-Paraffin: ESRP1 Antibody [NBP1-76536] - Human colon tissue with RBM35A antibody at 2.5 ug/mL.

Immunocytochemistry/Immunofluorescence: ESRP1 Antibody [NBP1-76536] -

Immunocytochemistry/Immunofluorescence: ESRP1 Antibody [NBP1-76536] - Human colon tissue cells with ESRP1 antibody at 20 ug/mL.
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Applications for ESRP1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ESRP1

RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.

Alternate Names

epithelial splicing regulatory protein 1, FLJ20171, RBM35A, RMB35A, RNA binding motif protein 35A, RNA-binding motif protein 35A, RNA-binding protein 35A

Gene Symbol

ESRP1

UniProt

NP_001030087 (Human)

Additional ESRP1 Products

Product Documents for ESRP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ESRP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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