ETFA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49845

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-49845
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ETFA Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QVADYGIVADLFK corresponding to C-Terminus according to NP_001121188.1.

Epitope

QVADYGIVADLFK - C Terminus

Specificity

This antibody is expected to recognize both reported isoforms (NP_000117.1; NP_001121188.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ETFA Antibody

Western Blot: ETFA Antibody [NBP1-49845]

Western Blot: ETFA Antibody [NBP1-49845]

Western Blot: ETFA Antibody [NBP1-49845] - Analysis of ETFA in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-49845 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ETFA Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.05 - 0.2 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human, mouse and rat heart lysates (calculated MW of 30.0 kDa band according to NP_001121188.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ETFA

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alpha-ETF, electron transfer flavoprotein alpha-subunit, Electron transfer flavoprotein subunit alpha, mitochondrial, electron-transfer-flavoprotein, alpha polypeptide, EMA, GA2, glutaric aciduria II, MADD

Entrez Gene IDs

2108 (Human); 110842 (Mouse); 300726 (Rat)

Gene Symbol

ETFA

UniProt

NP_000117.1 (Human)

Additional ETFA Products

Product Documents for ETFA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ETFA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.