ETFA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52058

Novus Biologicals, part of Bio-Techne
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NBP1-52058
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ETFA Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KSPDTFVRTIYAGN corresponding to internal region according to NP_001121188.1.

Epitope

C-KSPDTFVRTIYAGN

Specificity

This antibody is expected to recognize both isoforms (NP_000117.1; NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ETFA Antibody

Western Blot: ETFA Antibody [NBP1-52058]

Western Blot: ETFA Antibody [NBP1-52058]

Western Blot: ETFA Antibody [NBP1-52058] - Analysis of ETFA (aa139-152) in Human Colon lysate (35 ug protein in RIPA buffer) using NBP1-52058 at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ETFA Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
In Western blot, ~30+35 kDa bands observed in Human Colon lysates. Calculated MW of 35.1 kDa according to NP_000117.1 and of 30.0 kDa according to NP_001121188.1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ETFA

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alpha-ETF, electron transfer flavoprotein alpha-subunit, Electron transfer flavoprotein subunit alpha, mitochondrial, electron-transfer-flavoprotein, alpha polypeptide, EMA, GA2, glutaric aciduria II, MADD

Entrez Gene IDs

2108 (Human); 110842 (Mouse); 300726 (Rat)

Gene Symbol

ETFA

UniProt

NP_000117.1 (Human)

Additional ETFA Products

Product Documents for ETFA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ETFA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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