ETFB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97524

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ETFB Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human ETFB fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ETFB Antibody

Western Blot: ETFB Antibody [NBP2-97524]

Western Blot: ETFB Antibody [NBP2-97524]

Western Blot: ETFB Antibody [NBP2-97524] - Anti-ETFB rabbit polyclonal antibody at 1:500 dilution. Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa
Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP2-97524]

Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP2-97524]

Immunocytochemistry/Immunofluorescence: ETFB Antibody [NBP2-97524] - Immunofluorescence staining of ETFB in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ETFB polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524]

Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524]

Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524] - Immunochemical staining of human ETFB in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
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Applications for ETFB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ETFB

ETFB is a is an electron-transfer-flavoprotein, (beta polypeptide), and a subcomponent of Electron transfer flavoprotein (ETF). ETF exists in the mitochondrial matrix as a heterodimer of 30-kD alpha subunits (ETFA) and 28-kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. This electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases and transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

Alternate Names

Beta-ETF, electron transfer flavoprotein beta subunit, electron transfer flavoprotein beta-subunit, electron transfer flavoprotein subunit beta, electron transfer flavoprotein, beta polypeptide, electron-transfer-flavoprotein, beta polypeptide, electron-transferring-flavoprotein, beta polypeptide, MADD

Gene Symbol

ETFB

Additional ETFB Products

Product Documents for ETFB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ETFB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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