ETHE1 Antibody (001)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90300
Recombinant Monoclonal Antibody
![Western Blot: ETHE1 Antibody (001) [NBP2-90300]](https://resources.bio-techne.com/images/products/ETHE1-Antibody-001-Western-Blot-NBP2-90300-img0001.jpg "Western Blot: ETHE1 Antibody (001) [NBP2-90300]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 001
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ETHE1 (Uniprot#: O95571; Leu13-Ala254).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ETHE1 Antibody (001)
Western Blot: ETHE1 Antibody (001) [NBP2-90300]
Western Blot: ETHE1 Antibody (001) [NBP2-90300] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:28 kDa![Immunoprecipitation: ETHE1 Antibody (001) [NBP2-90300]](https://resources.bio-techne.com/images/products/ETHE1-Antibody-001-Immunoprecipitation-NBP2-90300-img0002.jpg "Immunoprecipitation: ETHE1 Antibody (001) [NBP2-90300]")
Immunoprecipitation: ETHE1 Antibody (001) [NBP2-90300]
Immunoprecipitation: ETHE1 Antibody (001) [NBP2-90300] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-ETHE1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ETHE1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDaApplications for ETHE1 Antibody (001)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ETHE1
Alternate Names
D83198, EC 3.1.2.6, ethylmalonic encephalopathy 1, Ethylmalonic encephalopathy protein 1, Hepatoma subtracted clone one protein, HSCOEC 3.-, protein ETHE1, mitochondrial, YF13H12
Gene Symbol
ETHE1
Additional ETHE1 Products
Product Documents for ETHE1 Antibody (001)
Product Specific Notices for ETHE1 Antibody (001)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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