ETS2 Antibody

Name: ETS2 Antibody
Brand: Novus Biologicals
SKU: NBP2-14951

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14951

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Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ETS2 Antibodies »

Product Summary for ETS2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETS2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ETS2 Antibody

Immunocytochemistry/ Immunofluorescence: ETS2 Antibody [NBP2-14951]

Immunocytochemistry/Immunofluorescence: ETS2 Antibody [NBP2-14951] - Paraformaldehyde-fixed HeLa, using ETS2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: ETS2 Antibody [NBP2-14951]

Immunohistochemistry-Paraffin: ETS2 Antibody [NBP2-14951] - Rat fore brain. ETS2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Chromatin Immunoprecipitation: ETS2 Antibody [NBP2-14951] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PTHrP promoter.

Immunoprecipitation: ETS2 Antibody [NBP2-14951]

Immunoprecipitation: ETS2 Antibody [NBP2-14951] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 ug of ETS2 antibody 7.5% SDS-PAGE The immunoprecipitated ETS2 protein was detected by ETS2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Western Blot: ETS2 Antibody [NBP2-14951] -

Western Blot: ETS2 Antibody [NBP2-14951] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ETS2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: ETS2 Antibody [NBP2-14951] -

Western Blot: ETS2 Antibody [NBP2-14951] -Untreated (–) and treated (+) Raw 264.7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETS2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: ETS2 Antibody [NBP2-14951] -

Western Blot: ETS2 Antibody [NBP2-14951] - Untreated (-) and treated (+) Raw 264.7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ETS2 antibody (NBP2-14951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ETS2 Antibody [NBP2-14951] -

Western Blot: ETS2 Antibody [NBP2-14951] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ETS2 antibody (NBP2-14951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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Applications for ETS2 Antibody

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ETS2

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Alternate Names

ETS2IT1, human erythroblastosis virus oncogene homolog 210protein C-ets-2, oncogene ETS-2, v-ets avian erythroblastosis virus E2 oncogene homolog 2, v-ets avian erythroblastosis virus E26 oncogene homolog 2, v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)

Gene Symbol

ETS2

UniProt

P15036 (Human)

Additional ETS2 Products

Product Documents for ETS2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for ETS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.

ETS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14951

Key Product Details

Validated by

Species Reactivity

Validated:

Predicted:

Applications

Label

Antibody Source

Concentration

View all ETS2 Antibodies »

Product Summary for ETS2 Antibody

Immunogen

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for ETS2 Antibody

Immunocytochemistry/ Immunofluorescence: ETS2 Antibody [NBP2-14951]

Immunohistochemistry-Paraffin: ETS2 Antibody [NBP2-14951]

Immunoprecipitation: ETS2 Antibody [NBP2-14951]

Western Blot: ETS2 Antibody [NBP2-14951] -

Western Blot: ETS2 Antibody [NBP2-14951] -

Western Blot: ETS2 Antibody [NBP2-14951] -

Western Blot: ETS2 Antibody [NBP2-14951] -

Applications for ETS2 Antibody

Chromatin Immunoprecipitation (ChIP)

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: ETS2

Alternate Names

Gene Symbol

UniProt

Additional ETS2 Products

Product Documents for ETS2 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for ETS2 Antibody