ETV5/ERM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14950

Novus Biologicals, part of Bio-Techne
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NBP2-14950
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (98%), Porcine (98%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ETV5 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETV5/ERM. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ETV5/ERM Antibody

Western Blot: ETV5/ERM Antibody [NBP2-14950]

Western Blot: ETV5/ERM Antibody [NBP2-14950]

Western Blot: ETV5/ERM Antibody [NBP2-14950] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ETV5/ERM Antibody [NBP2-14950]

Immunocytochemistry/ Immunofluorescence: ETV5/ERM Antibody [NBP2-14950]

Immunocytochemistry/Immunofluorescence: ETV5/ERM Antibody [NBP2-14950] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERM protein stained by ERM antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um
Western Blot: ETV5/ERM Antibody [NBP2-14950]

Western Blot: ETV5/ERM Antibody [NBP2-14950]

Western Blot: ETV5/ERM Antibody [NBP2-14950] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
View 2 more images

Applications for ETV5/ERM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Application Notes
IHC-Fr Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ETV5

Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.

Long Name

ETS Translocation Variant 5

Alternate Names

ERM

Gene Symbol

ETV5

UniProt

P41161

Additional ETV5 Products

Product Documents for ETV5/ERM Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ETV5/ERM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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