Eukaryotic translation initiation factor 1 Antibody (OTI2B9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00702

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2B9

Concentration

1.32 mg/ml

View all Eukaryotic translation initiation factor 1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human EIF1 (NP_005792) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Eukaryotic translation initiation factor 1 Antibody (OTI2B9)

Western Blot: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) [NBP2-00702]

Western Blot: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) [NBP2-00702]

Western Blot: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Eukaryotic translation initiation factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eukaryotic translation initiation factor 1.
Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Eukaryotic translation initiation factor 1.
Immunohistochemistry-Paraffin: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) [NBP2-00702]

Immunohistochemistry-Paraffin: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) [NBP2-00702]

Immunohistochemistry-Paraffin: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Staining of paraffin-embedded Human lymph node tissue using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.
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Applications for Eukaryotic translation initiation factor 1 Antibody (OTI2B9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1.32 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Eukaryotic translation initiation factor 1

EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

A121Protein translation factor SUI1 homolog, eIF1, EIF-1, EIF1A, eukaryotic translation initiation factor 1, ISO1, Sui1iso1, SUI1sui1iso1

Entrez Gene IDs

10209 (Human)

Gene Symbol

EIF1

Additional Eukaryotic translation initiation factor 1 Products

Product Documents for Eukaryotic translation initiation factor 1 Antibody (OTI2B9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Eukaryotic translation initiation factor 1 Antibody (OTI2B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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