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Eukaryotic translation initiation factor 1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03239

Novus Biologicals, part of Bio-Techne
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NBP3-03239-100ul
NBP3-03239-20ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human Eukaryotic translation initiation factor 1 (NP_005792.1). MSAIQNLHSFDPFADASKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLVEIGLAKDDQLKVHGF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Eukaryotic translation initiation factor 1 AntibodyAzide and BSA Free [NBP3-03239]

Western Blot: Eukaryotic translation initiation factor 1 AntibodyAzide and BSA Free [NBP3-03239]

Western Blot: Eukaryotic translation initiation factor 1 Antibody [NBP3-03239] - Analysis of extracts of various cell lines, using Eukaryotic translation initiation factor 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: Blocking buffer: 3% nonfat dry milk in TBST.

Applications

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Eukaryotic translation initiation factor 1

EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

A121Protein translation factor SUI1 homolog, eIF1, EIF-1, EIF1A, eukaryotic translation initiation factor 1, ISO1, Sui1iso1, SUI1sui1iso1

Gene Symbol

EIF1

Additional Eukaryotic translation initiation factor 1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov